Nanomechanical Characterization of Amyloid Fibrils Using Single-Molecule Experiments and Computational Simulations
نویسندگان
چکیده
منابع مشابه
Revealing the bifurcation in the unfolding pathways of GFP by using single-molecule experiments and simulations.
Nanomanipulation of biomolecules by using single-molecule methods and computer simulations has made it possible to visualize the energy landscape of biomolecules and the structures that are sampled during the folding process. We use simulations and single-molecule force spectroscopy to map the complex energy landscape of GFP that is used as a marker in cell biology and biotechnology. By enginee...
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Mass spectrometry provides rapid and quantitative identification of protein species with relatively low sample consumption. The trend towards biological analysis at increasingly smaller scales, ultimately down to the volume of an individual cell, continues, and mass spectrometry with a sensitivity of a few to single molecules will be necessary. Nanoelectromechanical systems provide unparalleled...
متن کاملToward single-molecule nanomechanical mass spectrometry
Mass spectrometry provides rapid and quantitative identification of protein species with relatively low sample consumption. The trend towards biological analysis at increasingly smaller scales, ultimately down to the volume of an individual cell, continues, and mass spectrometry with a sensitivity of a few to single molecules will be necessary. Nanoelectromechanical systems provide unparalleled...
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Amyloid proteins aggregate into polymorphic fibrils that damage tissues of the brain, nerves, and heart. Experimental and computational studies have examined the structural basis and the nucleation of short fibrils, but the ability to predict and precisely quantify the stability of larger aggregates has remained elusive. We established a complete classification of fibril shapes and developed a ...
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ژورنال
عنوان ژورنال: Journal of Nanomaterials
سال: 2016
ISSN: 1687-4110,1687-4129
DOI: 10.1155/2016/5873695